Human p70 S6 Kinase beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2987
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p70 S6 Kinase beta
Ala2-Ser410
Accession # Q9UBS0
Ala2-Ser410
Accession # Q9UBS0
Specificity
Detects human p70 S6 Kinase beta in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human p70 S6 Kinase is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human p70 S6 Kinase beta Antibody
Detection of Human p70 S6 Kinase beta by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p70 S6 Kinase beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p70 S6 Kinase beta in MCF‑7 Human Cell Line.
p70 S6 Kinase beta was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human p70 S6 Kinase beta Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p70 S6 Kinase beta
Alternate Names
KLS, p70-beta, RPS6KB2, STK14B
Gene Symbol
RPS6KB2
UniProt
Additional p70 S6 Kinase beta Products
Product Documents for Human p70 S6 Kinase beta Antibody
Product Specific Notices for Human p70 S6 Kinase beta Antibody
For research use only
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