Human PADI2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7257
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PADI2
Met1-Ala115
Accession # Q9Y2J8
Met1-Ala115
Accession # Q9Y2J8
Specificity
Detects human PADI2 in direct ELISAs.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PADI2 Antibody
PADI2 in Human Brain.
PADI2 was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex) using Sheep Anti-Human PADI2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7257) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PADI2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex)
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's brain (cortex)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PADI2
PADI2 (Protein-arginine deiminase type 2; also PAD2, PDI2 and PAD-H19) is a 72-76 kDa member of the PAD family of proteins. It is widely expressed, being found in macrophages, astrocytes, select neurons, skeletal muscle and bronchial epithelial cells, and keratinocytes. PADI2 catalyzes the Ca++-dependent conversion of guanidino groups (NH2-C[=NH]-NH−) to ureido groups (NH2-C[=O]-NH-) on peptidylArg, a posttranslational process known as citrullination. Human PADI2 is 665 amino acids (aa) in length. It contains an N-terminal PAD domain (aa 1-114), a middle PAD-M domain (aa 116-274), and a catalytic region (aa 279-665). Over aa 1-115, human PADI2 shares 90% aa sequence identity with mouse PADI2.
Long Name
Peptidyl Arginine Deiminase type II
Alternate Names
PAD-H19, PAD2, PDI2, Peptidylarginine Deiminase II
Gene Symbol
PADI2
UniProt
Additional PADI2 Products
Product Documents for Human PADI2 Antibody
Product Specific Notices for Human PADI2 Antibody
For research use only
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