Human PAM Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4837
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PAM
Phe21-Val817
Accession # P19021
Phe21-Val817
Accession # P19021
Specificity
Detects human PAM in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human PAM Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human PAM
Sample: Recombinant Human PAM
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAM
PAM (Peptidyl-Gly alpha-amidating monooxygenase) is a dual-function, 95‑110 kDa protein that belongs to both the lyase and monooxygenase enzyme families. It is expressed in a variety of endocrine and exocrine glands and catalyzes the conversion of C‑terminal Gly into amidated residues. Human ProPAM is a 953 amino acid (aa) type I transmembrane glycoprotein. It contains an 833 aa extracellular domain (ECD) (aa 31‑863) and an 86 aa cytoplasmic tail. The ECD shows one PHM/monooxygenase region (aa 40‑347) and a PAL/lyase domain (aa 495‑817). Both regions are enzymatically active. Alternative splicing may generate a soluble form (aa 31‑828), and remove aa 388‑494 that link the two embedded enzyme segments. Over aa 21‑817, human PAM is 91% aa identical to mouse PAM.
Long Name
Peptidylglycine Alpha-Amidating Monooxygenase
Alternate Names
PAL, PHM
Gene Symbol
PAM
UniProt
Additional PAM Products
Product Documents for Human PAM Antibody
Product Specific Notices for Human PAM Antibody
For research use only
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