Human PAMR1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6517
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PAMR1
Leu22-Lys737
Accession # NP_056245
Leu22-Lys737
Accession # NP_056245
Specificity
Detects human PAMR1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PAMR1 Antibody
Detection of Human PAMR1 by Western Blot.
Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAMR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAMR1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PAMR1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: U2OS human osteosarcoma cell line
Sample: U2OS human osteosarcoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAMR1
PAMR1 (Peptidase domain associated with muscle regeneration 1; also RAMP/ regeneration-associated muscle protease homolog) is a secreted, 80 kDa (predicted) member of the peptidase S1 family of enzymes. It is expressed by skeletal muscle, and appears to mediate skeletal muscle regeneration. Due to the lack of a catalytic serine at amino acid (aa) 672, PAMR1 demonstrates no enzyme activity. Mature PAMR1 is 716 aa in length (aa 22-737). It contains a Cys-rich segment (aa 42-94), one CUB domain (aa 128-234), an EGF-like region (aa 235-272), two SUSHI domains (aa 278-444) and a nonfunctional peptidase S1 domain (aa 445-737). There is one 17 aa deletion after Glu276. Over aa 21-737, human PAMR1 shares 88% aa identity with mouse PAMR1.
Long Name
Peptidase Domain Containing Associated with Muscle Regeneration 1
Alternate Names
DKFZP586H2123, RAMP
Gene Symbol
PAMR1
UniProt
Additional PAMR1 Products
Product Documents for Human PAMR1 Antibody
Product Specific Notices for Human PAMR1 Antibody
For research use only
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