Human PAR2 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB3949A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 344222
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human PAR2
Accession # P55085
Accession # P55085
Specificity
Detects human PAR2. Stains human PAR2 transfectants but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PAR2 APC-conjugated Antibody
Detection of PAR2 in HT-29 Human Cell Line by Flow Cytometry.
HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human PAR2 APC-conjugated Monoclonal Antibody (Catalog # FAB3949A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human PAR2 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HT-29 human colon adenocarcinoma cell line
Sample: HT-29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PAR2
Protease-Activated Receptor 2 (PAR2) is a protease activated 7-transmembrane G-protein-coupled receptor. Human PAR2 contains a cleavage site for trypsin, mast cell tryptase or coagulation factor VIIa or Xa, 11 amino acids (aa) C-terminal to the signal sequence. Cleavage creates a tethered ligand that activates the 361 aa receptor. PAR2 is expressed in kidney, pancreas, stomach, intestine, airway, skin, bladder and brain; activation stimulates release of inflammatory and nociceptive mediators. PAR2 is downregulated by ubiquination, endocytosis and degradation. Mature human PAR2 shows 78% amino acid identity with mouse PAR2 over the extracellular portions.
Long Name
Protease-Activated Receptor 2
Alternate Names
CFIIRL1, F2RL1, GPCR11
Gene Symbol
F2RL1
UniProt
Additional PAR2 Products
Product Documents for Human PAR2 APC-conjugated Antibody
Product Specific Notices for Human PAR2 APC-conjugated Antibody
For research use only
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