Human PARD3/Par3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8030

R&D Systems, part of Bio-Techne
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MAB8030
MAB8030-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry

Cited:

Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 833517

View all PARD3/Par3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PARD3/Par3
Tyr451-Glu555
Accession # Q8TEW0

Specificity

Detects human PARD3/Par3 in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human PARD3b/Par3b is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PARD3/Par3 Antibody

PARD3/Par3 antibody in HEK293 Human Cell Line by Immunocytochemistry (ICC).

PARD3/Par3 in HEK293 Human Cell Line.

PARD3/Par3 was detected in immersion fixed HEK293 human embryonic kidney cell line using Mouse Anti-Human PARD3/Par3 Monoclonal Antibody (Catalog # MAB8030) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PARD3/Par3 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PARD3/Par3

PARD3 (partitioning defective 3 homolog), also called Par3, is a widely expressed adapter protein involved in asymmetrical cell division, cell polarization, and tight junction formation. It forms a cell polarity complex with Par6 and aPKC (atypical protein kinase C), linking to PTEN (phosphatase and tensin homolog) in polarized epithelia and VE-Cadherin in polarized endothelia. The region used as an immunogen, human PARD3 amino acids (aa) 451-555 (of 1356 aa in full-length PARD3) shares 97% and 96% aa sequence identity with mouse and rat PARD3, respectively. It includes the second of three PDZ domains, a region which is involved in heterophilic interactions and is present within all 10 reported PARD3 isoforms that vary in length from 988 aa to 1356 aa.

Long Name

Par-3 Partitioning Defective 3 Homolog

Alternate Names

ASIP, Baz, Bazooka, PAR3, PAR3A, SE2-5L16, SE2-5LT1, SE2-5T2

Entrez Gene IDs

56288 (Human); 93742 (Mouse); 81918 (Rat)

Gene Symbol

PARD3

UniProt

Q8TEW0

Additional PARD3/Par3 Products

Product Documents for Human PARD3/Par3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PARD3/Par3 Antibody

For research use only

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