Human Park7/DJ-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3995
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Park7/DJ-1
Met1-Asp189
Accession # Q99497
Met1-Asp189
Accession # Q99497
Specificity
Detects human Park7/DJ-1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Park7/DJ-1 Antibody
Detection of Human Park7/DJ-1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Park7/DJ-1 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Park7/DJ‑1 in HeLa Human Cell Line.
Park7/DJ-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Park7/DJ-1 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Park7/DJ-1 at approximately 28 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Park7/DJ-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3995) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Park7/DJ-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus)
Knockout Validated
Park7/DJ‑1
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
Park7/DJ‑1 knockout HEK293T cell line.
Simple Western
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and A172 human glioblastoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Park7/DJ-1
Park7, also known as DJ-1, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ-1 superfamily of highly conserved proteins that function as protein chaperones, catalases, proteases and kinases. Park7 is widely expressed in the brain as well as in peripheral tissues. It exists as a homodimer that can be localized in the cytoplasm, nucleus and mitochondria. Park7 is a redox-sensitive protein that has been ascribed various functions including that as a redox sensor and antioxidant protein. Mutations in Park7 are associated with a small percentage of hereditary early onset Parkinson’s disease.
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
UniProt
Additional Park7/DJ-1 Products
Product Documents for Human Park7/DJ-1 Antibody
Product Specific Notices for Human Park7/DJ-1 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...