Human PAUF/ZG16B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7777
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 817310
Product Specifications
Immunogen
E. coli-derived recombinant human PAUF/ZG16B
Gly53-Arg208
Accession # Q96DA0
Gly53-Arg208
Accession # Q96DA0
Specificity
Detects human PAUF/ZG16B in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PAUF/ZG16B Antibody
Detection of Human PAUF/ZG16B by Western Blot.
Western blot shows lysates of human placenta tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PAUF/ZG16B at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PAUF/ZG16B in Human Pancreas Cancer Tissue.
PAUF/ZG16B was detected in immersion fixed paraffin-embedded sections of human pancreas cancer tissue using Mouse Anti-Human PAUF/ZG16B Monoclonal Antibody (Catalog # MAB7777) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membranes of cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PAUF/ZG16B Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human pancreas cancer tissue
Western Blot
1 µg/mL
Sample: Human placenta tissue and human prostate tissue
Sample: Human placenta tissue and human prostate tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAUF/ZG16B
Long Name
Pancreatic Adenocarcinoma Upregulated Factor
Alternate Names
EECP, HRPE773, JCLN2, LOC124220, PRO1567, ZG16B
Entrez Gene IDs
124220 (Human)
Gene Symbol
ZG16B
UniProt
Additional PAUF/ZG16B Products
Product Documents for Human PAUF/ZG16B Antibody
Product Specific Notices for Human PAUF/ZG16B Antibody
For research use only
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