Human Pax3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11430
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 274206
Product Specifications
Immunogen
E. coli-derived recombinant human PAX-3
Met1-Leu215
Accession # P23760
Met1-Leu215
Accession # P23760
Specificity
Detects human PAX-3 in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pax3 Antibody
Detection of Pax3 in Human Skin.
Pax3 was detected in immersion fixed paraffin-embedded sections of Human Skin using Mouse Anti-Human Pax3 Monoclonal Antibody (Catalog # MAB11430) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Pax3 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Skin
Sample: Immersion fixed paraffin-embedded sections of Human Skin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pax3
Long Name
Paired Box Gene 3 [Waardenburg Syndrome 1]
Alternate Names
HUP2, WS1
Gene Symbol
PAX3
UniProt
Additional Pax3 Products
Product Documents for Human Pax3 Antibody
Product Specific Notices for Human Pax3 Antibody
For research use only
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