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Human PDGF R alpha (Research Grade Olaratumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10352

Recombinant Monoclonal Antibody For Research Use Only
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu136

Product Specifications

Immunogen

Human PDGF R alpha

Specificity

Detects Human PDGF R alpha in direct ELISAs.This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Olaratumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images

Detection of PDGF R alpha antibody in U-118-MG human glioblastoma cell line antibody by Flow Cytometry.

Detection of PDGF R alpha in U-118-MG human glioblastoma cell line by Flow Cytometry.

U-118-MG human glioblastoma cell line was stained with Human Anti-Human PDGF R alpha (Research Grade Olaratumab Biosimilar) Monoclonal Antibody (Catalog # MAB10352, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: U-118-MG human glioblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R alpha

Olaratumab is a biosimilar directed against human PDGF R alpha.

Long Name

Platelet-derived Growth Factor Receptor alpha

Alternate Names

CD140a, PDGFR alpha, PDGFRA

Entrez Gene IDs

5156 (Human); 18595 (Mouse)

Gene Symbol

PDGFRA

Additional PDGF R alpha Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices

For research use only

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