Human PDX-1/IPF1 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAF2419
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Flow Cytometry
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PDX‑1/IPF1
Ala91-Arg283
Accession # P52945
Ala91-Arg283
Accession # P52945
Specificity
Detects human PDX‑1/IPF1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PDX-1/IPF1 Biotinylated Antibody
PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells.
PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells using Goat Anti-Human PDX-1/IPF1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PDX-1/IPF1 Biotinylated Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to pancreatic progenitor cells
Western Blot
0.1 µg/mL
Sample: Recombinant Human PDX-1/IPF1
Sample: Recombinant Human PDX-1/IPF1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDX-1/IPF1
Long Name
Pancreas/Duodenum Homeobox-1
Alternate Names
IDX1, IPF1
Gene Symbol
PDX1
UniProt
Additional PDX-1/IPF1 Products
Product Documents for Human PDX-1/IPF1 Biotinylated Antibody
Product Specific Notices for Human PDX-1/IPF1 Biotinylated Antibody
For research use only
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