Human PDZD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7670
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 787924
Product Specifications
Immunogen
E. coli-derived recombinant human PDZD2
Ser2489-Ser2839
Accession # O15018
Ser2489-Ser2839
Accession # O15018
Specificity
Detects human PDZD2 in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant mouse PDZD2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PDZD2 Antibody
Detection of Human PDZD2 by Western Blot.
Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PDZD2 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDZD2 in PC‑3 Human Cell Line.
PDZD2 was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human PDZD2 Monoclonal Antibody (Catalog # MAB7670) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PDZD2 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line
Sample: Immersion fixed PC‑3 human prostate cancer cell line
Western Blot
2 µg/mL
Sample: Human prostate tissue
Sample: Human prostate tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDZD2
Long Name
PDZ Domain Containing 2
Alternate Names
AIPC, KIAA0300, Papin, Pin1
Gene Symbol
PDZD2
UniProt
Additional PDZD2 Products
Product Documents for Human PDZD2 Antibody
Product Specific Notices for Human PDZD2 Antibody
For research use only
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