Human Pepsinogen A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7525
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 762015
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A4/PGA4
Ile16-Ala388
Accession # P00790
Ile16-Ala388
Accession # P00790
Specificity
Detects human Pepsinogen A4/PGA4 in direct ELISAs. Human PGA3 and PGA5 reactivity has not been demonstrated but is likely due to their high homology with human PGA4. In direct ELISAs, no cross-reactivity with recombinant human (rh) Pepsinogen C, rhCathepsin D, or rhCathepsin E is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Pepsinogen A Antibody
Detection of Human Pepsinogen A by Western Blot.
Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Pepsinogen A Monoclonal Antibody (Catalog # MAB7525) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Pepsinogen A at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Pepsinogen A in Human Stomach.
Pepsinogen A was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Pepsinogen A Monoclonal Antibody (Catalog # MAB7525) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Pepsinogen A Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach
Sample: Immersion fixed paraffin-embedded sections of human stomach
Western Blot
0.2 µg/mL
Sample: Human stomach tissue
Sample: Human stomach tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pepsinogen A
Alternate Names
EC 3.4.23, EC 3.4.23.1, FLJ58952, FLJ77962, pepsin A, pepsinogen 4, group I (pepsinogen A), pepsinogen A4, PGA3, PGA5
Entrez Gene IDs
643847 (Human)
Gene Symbol
PGA4
UniProt
Additional Pepsinogen A Products
Product Documents for Human Pepsinogen A Antibody
Product Specific Notices for Human Pepsinogen A Antibody
For research use only
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