Human Pepsinogen A5/PGA5 Antibody

Name: Human Pepsinogen A5/PGA5 Antibody
Brand: R&D Systems
SKU: AF8457

R&D Systems, part of Bio-Techne | Catalog # AF8457

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pepsinogen A5/PGA5
Met1-Ala388
Accession # P0DJD7

Specificity

Detects human Pepsinogen A5/PGA5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Pepsinogen A5/PGA5 Antibody

Detection of Human Pepsinogen A5/PGA5 by Western Blot.

Western blot shows lysates of human stomach tissue. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pepsinogen A5/PGA5 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Pepsinogen A5/PGA5 by Simple Western^TM.

Simple Western lane view shows lysates of human stomach tissue and human stomach cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for Pepsinogen A5/PGA5 at approximately 48 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human Pepsinogen A5/PGA5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8457) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Pepsinogen A5/PGA5 Antibody

Application

Recommended Usage

Simple Western

20 µg/mL
Sample: Human stomach tissue and Human stomach cancer tissue

Western Blot

0.1 µg/mL
Sample: Human stomach tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pepsinogen A5/PGA5

Pepsins are aspartic proteases that are synthesized in the gastric mucosa and secreted into the stomach. They are released as zymogens called pepsinogens and then converted to active pepsins by the acidic pH of gastric juices (1). PGA-3, PGA-4, and PGA-5 are isozymogens of human Pepsinogen A, which differ in amino acid sequence by 2-4 residues (2). This recombinant human Pepsinogen A corresponds to PGA-4. Pepsins have optimal activity under conditions of acidic pH and are inhibited by pepstatin. Pepsin A has broad substrate specificity, but preferentially cleaves peptide bonds involving aromatic and aliphatic amino acids.

References

Athauda, S.B. et al. (1989) J. Biochem. 106:920.
Zwiers, A. et al. (1994) Clin. Nephrol. 41:153.

Alternate Names

PGA5

Entrez Gene IDs

5222 (Human)

Gene Symbol