Human Perilipin-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB76641
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 779103
Product Summary for Human Perilipin-3 Antibody
Immunogen
E. coli-derived recombinant human Perilipin‑3
Met1-Lys434
Accession # O60664
Met1-Lys434
Accession # O60664
Specificity
Detects human Perilipin‑3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant
human (rh) Perilipin-2 or rhPerilipin-5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Perilipin-3 Antibody
Detection of Human Perilipin‑3 by Western Blot.
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB76641) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-3 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Perilipin‑3 in Human Placenta.
Perilipin-3 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Perilipin-3 Monoclonal Antibody (Catalog # MAB76641) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Perilipin-3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta
Sample: Immersion fixed paraffin-embedded sections of human placenta
Western Blot
0.25 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Sample: Daudi human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Perilipin-3
Alternate Names
M6PRBP1, Perilipin3, PLIN3, PP17, TIP47
Gene Symbol
PLIN3
UniProt
Additional Perilipin-3 Products
Product Documents for Human Perilipin-3 Antibody
Product Specific Notices for Human Perilipin-3 Antibody
For research use only
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