Human Perilipin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6615
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Thr8-Ala145
Accession # O60240
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Perilipin Antibody
Detection of Human Perilipin by Western Blot.
Western blot shows lysates of human heart (adipose) tissue. PVDF Membrane was probed with 0.25 µg/mL of Human Perilipin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6615) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Perilipin at approximately 55-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Perilipin Antibody
Western Blot
Sample: Human heart (adipose) tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Perilipin
Perilipin (Perilipin-1/A; also PLIN1 and Lipid droplet-associated protein) is a 62 kDa constitutively phosphorylated protein that belongs to the PAT (Perilipin/Adipophilin/TIP47) family of molecules. Found primarily in mature adipocytes, perilipin-1 interacts with lipid-coat proteins at the edge of the fat droplet and blocks lipase activity. Upon phosphorylation, perilipin-1 becomes a 65-67 kDa molecule that likely promotes the dispersion of docking molecules and provides a scaffold for lipase at the lipid droplet surface. Human perilipin-1 is 522 amino acids (aa) in length. It contains a lipid droplet targeting region (aa 233-364) that contains a polyGlu segment (aa 307-316). Phosphorylation at the N-terminus is necessary for lipase interaction with lipid. One 50-54 kDa human splice variant (perilipin-B) is reported that shows an 85 aa substitution for aa 404-522. This apparently interacts with cell membrane triglycerides. Over aa 8-145, human perilipin-1/A shares 97% aa identity with mouse perilipin-1.
Alternate Names
Gene Symbol
UniProt
Additional Perilipin Products
Product Documents for Human Perilipin Antibody
Product Specific Notices for Human Perilipin Antibody
For research use only