Human PGLYRP1/PGRP-S Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB25901

R&D Systems, part of Bio-Techne
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MAB25901-100
MAB25901-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1040002

View all PGLYRP1/PGRP-S Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived human PGLYRP1/PGRP-S
Gln22-Pro196
Accession # O75594

Specificity

Detects human PGLYRP1/PGRP-S in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human PGLYRP1/PGRP-S Antibody

PGLYRP1/PGRP-S in Human Spleen.

PGLYRP1/PGRP-S was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human PGLYRP1/PGRP-S Monoclonal Antibody (Catalog # MAB25901) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human PGLYRP1/PGRP-S Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PGLYRP1/PGRP-S

The human PGRP family is comprised of four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP-L, PGRP-I alpha, PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 576 amino acids (aa) in length, while PGRP-I alpha and I beta are (I) intermediate in length at 341 aa and 373 aa, respectively, and PGRP-S is the shortest at 196 aa in length (3, 4). All human PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Human PGRP-S, the first described member of the family, is a 28 kDa secreted glycoprotein associated with neutrophils (4). The mature molecule is 175 aa in length and contains three variably-sized peptide-carbohydrate recognition sequences of 15 aa, 29 aa and 49 aa, respectively. Human PGRP-S is 72%, 71% and 70% aa identical to mouse, bovine and rat mature PGRP-S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with non-pathogenic bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The longer three PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C-termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C-terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).

References

  1. Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
  2. Steiner, H. (2004) Immunol. Rev. 198:83.
  3. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
  4. Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
  5. Dziarski, R. et al. (2003) Blood 102:689.

Long Name

Peptidoglycan Recognition Protein Short/Peptidoglycan Recognition Protein 1

Alternate Names

PGRP-S, PGRPS, TAG7, TNFSF3L

Entrez Gene IDs

8993 (Human); 21946 (Mouse)

Gene Symbol

PGLYRP1

UniProt

O75594

Additional PGLYRP1/PGRP-S Products

Product Documents for Human PGLYRP1/PGRP-S Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human PGLYRP1/PGRP-S Antibody

For research use only

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