Human Phospho-IRS1 (Y1179) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7455
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 744353
Product Specifications
Immunogen
Phosphopeptide containing the human IRS1 Y1179 site
Specificity
Detects Human Phospho-IRS1 (Y1179) in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Phospho-IRS1 (Y1179) Antibody
Detection of Human Phospho-IRS1 (Y1179) by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1 µg/mL Insulin for 5 minutes and 300 U/mL CIP for 1 hour. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-IRS1 (Y1179) Monoclonal Antibody (Catalog # MAB7455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-IRS1 (Y1179) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-IRS1 (Y1179) in MCF‑7 Human Cell Line.
IRS1 phosphorylated at Y1179 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human Phospho-IRS1 (Y1179) Monoclonal Antibody (Catalog # MAB7455) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-IRS1 (Y1179) Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Insulin and CIP
Sample: MCF‑7 human breast cancer cell line treated with Insulin and CIP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IRS1
Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate intermediate between the insulin and IGF-I receptor, and downstream signaling modulators. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Human IRS1 is 1242 amino acids (aa) in length. It contains a PH (pleckstrin homology) domain (aa 12-115), followed by a PTB (phosphotyrosine-binding) domain (aa 160-263) and ten PEST (Pro/Glu/Ser/Thr) regions (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Insulin receptor-mediated phosphorylation of human IRS1 on Y1179, or its murine equivalent, Y1172, mediates IRS-1 interaction with SH2 domains such as that of the Src family kinase, Fyn, or the tyrosine phosphatase, SHP-2.
Long Name
Insulin Receptor Substrate 1
Alternate Names
HIRS-1, insulin receptor substrate 1, IRS-1
Gene Symbol
IRS1
Additional IRS1 Products
Product Documents for Human Phospho-IRS1 (Y1179) Antibody
Product Specific Notices for Human Phospho-IRS1 (Y1179) Antibody
For research use only
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