Human Phospho-PLC-gamma 1 (Y783) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7454
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 764518
Product Summary for Human Phospho-PLC-gamma 1 (Y783) Antibody
Immunogen
Phosphopeptide containing the human PLC-gamma 1 Y783 site
Specificity
Detects human Phospho-PLC‑ gamma1 (Y783) in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Phospho-PLC-gamma 1 (Y783) Antibody
Detection of Human Phospho-PLC‑ gamma1 (Y783) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-PLC- gamma1 (Y783) Monoclonal Antibody (Catalog # MAB7454) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Phospho-PLC- gamma1 (Y783) at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-PLC-gamma 1 (Y783) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV)
Sample: Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 1
Long Name
Phospholipase C gamma 1
Alternate Names
PLCG1, PLCgamma 1, PPLCA
Gene Symbol
PLCG1
Additional PLC-gamma 1 Products
Product Documents for Human Phospho-PLC-gamma 1 (Y783) Antibody
Product Specific Notices for Human Phospho-PLC-gamma 1 (Y783) Antibody
For research use only
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