Human PIBF1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5559
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PIBF1
Glu19-Ala419
Accession # Q8WXW3
Glu19-Ala419
Accession # Q8WXW3
Specificity
Detects human PIBF1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PIBF1 Antibody
Detection of Human PIBF1 by Western Blot.
Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and human breast cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PIBF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5559) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for PIBF1 at approximately 98 kDa and 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PIBF1 in Human Breast Cancer Tissue.
PIBF1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Sheep Anti-Human PIBF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5559) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human PIBF1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Western Blot
1 µg/mL
Sample: JEG-3 human epithelial choriocarcinoma cell line and human breast cancer tissue
Sample: JEG-3 human epithelial choriocarcinoma cell line and human breast cancer tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIBF1
PIBF-1 (Progesterone induced blocking factor 1) is a 90-92 kDa progesterone-inducible molecule initially isolated from the lymphocytes of pregnant women. It is produced by lymphocytes plus villous trophoblast cells, and modulates the activity of cytotoxic NK cells. Human PIBF-1 is 757 amino acids (aa) in length. It contains a possible signal sequence (aa 1-26), two Leu-zippers (aa 311-323 and 643-664), two NLSs (aa 282-285 and 567-573), a bZIP sequence (aa 574-614), and an ER retention motif (aa 752-757). PIBF-1 is found in the nucleus, cytoplasm, and circulation, the result of multiple splice variants. SDS-page shows 90 kDa, 80-83 kDa, 48-52 kDa, 34-35 kDa, and 10-12 kDa isoforms. The 90 kDa form is nuclear and full-length, while the 34-35 kDa form is secreted and likely represents aa 1-223 spliced to aa 683-757. There is a 50-55 kDa form that is apparently intracellular, possibly bioactive and likely represents the N-terminus of the molecule. Over aa 19‑419, human PIBF-1 shares 88% aa identity with mouse PIBF-1.
Long Name
Progesterone Immunomodulatory Binding Factor 1
Alternate Names
C13orf24, chromosome 13 open reading frame 24, KIAA1008, PIBF, progesterone immunomodulatory binding factor 1, progesterone-induced blocking factor 1, progesterone-induced-blocking factor 1, RP11-505F3.1
Gene Symbol
PIBF1
UniProt
Additional PIBF1 Products
Product Documents for Human PIBF1 Antibody
Product Specific Notices for Human PIBF1 Antibody
For research use only
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