In a functional ELISA, Recombinant Human PILR-a (Catalog # 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding.

Detection of PILR- alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human PILR- a or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PILR- a Monoclonal Antibody (Catalog # MAB64842) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050).

Applications for Human PILR-alpha Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with Human PILR- alpha and eGFP

Neutralization

In a functional ELISA, 1-5 μg/mL of this antibody will block 50% of the binding of 2 μg/mL Recombinant Human PILR‑ alpha (Catalog # 6484-PR) to immobilized recombinant human PANP Fc Chimera coated at 5 μg/mL (100 µL/well).

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PILR-alpha

PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14⁺CD1a^- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.

Long Name

Paired-Ig-likeType 2 Receptor alpha

Alternate Names

FDF03, PILRA, PILRalpha

Entrez Gene IDs

29992 (Human); 231805 (Mouse); 288568 (Rat)

Gene Symbol

PILRA

UniProt

Q9UKJ1 (Human)

Additional PILR-alpha Products

Product Documents for Human PILR-alpha Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PILR-alpha Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human PILR-alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB64842

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

View all PILR-alpha Antibodies »

Product Summary for Human PILR-alpha Antibody

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human PILR-alpha Antibody

PILR-alpha binding to PANP blocked by PILR-alpha Antibody.