Human PILR-alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB64842
Recombinant Monoclonal Antibody.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, Neutralization
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2175B
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PILR‑ alpha with a C-terminal 6-His tag
Gln20-Thr196
Accession # Q9UKJ1
Gln20-Thr196
Accession # Q9UKJ1
Specificity
Detects human PILR‑ alpha in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human PILR-alpha Antibody
PILR-alpha binding to PANP blocked by PILR-alpha Antibody.
In a functional ELISA, Recombinant Human PILR-a (Catalog # 6484-PR) binds to immobilized recombinant human PANP Fc Chimera coated at 5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PILR-a Monoclonal Antibody (Catalog # MAB64842). At 1-5 µg/mL, this antibody will block 50% of the binding.
Detection of PILR- alpha R in HEK293 Human Cell Line Transfected with Human PILR-alpha and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human PILR- a or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PILR- a Monoclonal Antibody (Catalog # MAB64842) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050).Applications for Human PILR-alpha Antibody
Application
Recommended Usage
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PILR- alpha and eGFP
Sample: HEK293 Human Cell Line Transfected with Human PILR- alpha and eGFP
Neutralization
In a functional ELISA, 1-5 μg/mL of this antibody will block 50% of the binding of 2 μg/mL Recombinant Human PILR‑ alpha (Catalog # 6484-PR) to immobilized recombinant human PANP Fc Chimera coated at 5 μg/mL (100 µL/well).
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PILR-alpha
PILR-alpha (paired immunoglobulin-like, type 2 receptor alpha) is a 44-50 kDa type I transmembrane (TM) paired receptor glycoprotein that belongs to the Ig Superfamily. It is expressed by monocytes, macrophages, CD14+CD1a- DC and retinal pigment cells, and is known to bind to CD99 and PANP. PILR-alpha acts as a receptor for HSV and serves as a negative immunomodulator that contains an ITIM. Mature human PILR-alpha is 284 amino acids (aa) in length. It contains one V-type Ig-like domain in its extracellular region (aa 32-150), and two ITIMs in its cytoplasmic domain (aa 267-272 and 296-301). There are multiple potential splice variants. One is TM and possesses a 35 aa substitution for aa 264-303, while others are soluble, and show a deletion of aa 152-224 that may be coupled to the 35 aa substitution noted above, or simply exhibit a 24 aa substitution for aa 152-303. Over aa 20-196, human PILR-alpha shares only 42% aa identity with mouse PILR-alpha, and 89% aa identity with human PILR-beta.
Long Name
Paired-Ig-likeType 2 Receptor alpha
Alternate Names
FDF03, PILRA, PILRalpha
Gene Symbol
PILRA
UniProt
Additional PILR-alpha Products
Product Documents for Human PILR-alpha Antibody
Product Specific Notices for Human PILR-alpha Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...