Human PIWIL4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6316
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 626001
Product Specifications
Immunogen
E. coli-derived recombinant human PIWIL4
Ser102-Gln237
Accession # Q7Z3Z4
Ser102-Gln237
Accession # Q7Z3Z4
Specificity
Detects human PIWIL4 in direct ELISAs. In this format, no cross-reactivity with recombinant human (rh) PIWIL2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PIWIL4 Antibody
PIWIL4 in BG01V Human Stem Cells.
PIWIL4 was detected in immersion fixed BG01V human embryonic stem cells using Human PIWIL4 Monoclonal Antibody (Catalog # MAB6316) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PIWIL4 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PIWIL4
PIWI-like protein 4 (PIWIL4) is a 97 kDa member of the argonaute family of proteins and the PIWI subfamily. Human PIWIL4 is 852 amino acids (aa) in length and contains one PAZ domain (aa 268-384) and one PIWI domain (aa 546-838). There are three isoforms for human PIWIL4. Isoform 1 is the standard protein. Isoform 2 has a two aa substitution for aa 1-29 in isoform 1 and a deletion of aa 523-852. Isoform 3 has a deletion of aa 1-69 and a deletion of aa 523-852. Human PIWIL4 shares 77% aa sequence identity with mouse and rat PIWIL4. PIWIL4 plays a central role in spermatogenesis.
Additional PIWIL4 Products
Product Documents for Human PIWIL4 Antibody
Product Specific Notices for Human PIWIL4 Antibody
For research use only
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