Human PKC delta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4585
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKC delta
His549-Asp676
Accession # Q05655
His549-Asp676
Accession # Q05655
Specificity
Detects human PKC delta in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PKC delta Antibody
Detection of Human PKC delta by Western Blot.
Western blot shows lysates of LNCaP human prostate cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCd at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human PKC delta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: LNCaP human prostate cancer cell line and HT‑29 human colon adenocarcinoma cell line
Sample: LNCaP human prostate cancer cell line and HT‑29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC delta
PKC delta (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKC delta is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aa's 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKC delta is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKC delta entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKC delta at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKC delta has been noted. Over aa 549‑676, human PKC delta is 85% aa identical to mouse PKC delta.
Long Name
Protein Kinase C delta
Alternate Names
PRKCD
Entrez Gene IDs
5580 (Human)
Gene Symbol
PRKCD
UniProt
Additional PKC delta Products
Product Documents for Human PKC delta Antibody
Product Specific Notices for Human PKC delta Antibody
For research use only
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