Human PKC zeta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6666
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human
Applications
Knockout Validated, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKC zeta
Ile454-Val592
Accession # Q05513
Ile454-Val592
Accession # Q05513
Specificity
Detects human PKC zeta in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human PKC zeta Antibody
Detection of Human PKC zeta by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, MCF-7 human breast cancer cell line, and human lung tissue. PVDF Membrane was probed with 1 µg/mL of Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC zeta at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human PKC zeta Specificity by Using Knockout Cell Line.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and PKC zeta knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKC zeta at approximately 75 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human PKC zeta by Simple WesternTM.
Simple Western shows lysates of MCF-7 human breast cancer cell line, loaded at 0.5 mg/ml. A specific band was detected for PKC zeta at approximately 79 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human PKC zeta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6666). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.Applications for Human PKC zeta Antibody
Application
Recommended Usage
Knockout Validated
PKC zeta
is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in
PKC zeta knockout HEK293T cell line.
Simple Western
10 µg/mL
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: 293T human embryonic kidney cell line, MCF‑7 human breast cancer cell line, and human lung tissue
Sample: 293T human embryonic kidney cell line, MCF‑7 human breast cancer cell line, and human lung tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC zeta
PKC zeta (protein kinase C-zeta, EC=2.7.11.13), also called PKC2, is a 592 amino acid (aa), 79 kDa member of the atypical PKC (aPKC) subfamily of Ser/Thr protein kinases. aPKC proteins can be activated by phorbol esters, but are insensitive to calcium and diacylglycerol. PKC zeta regulates cell polarity in early development, and is necessary for cytokine-induced chemotaxis of macrophages and cancer cells, chemokine-induced adhesion of neutrophils, and polarization of Th2 cells. It is activated by PDK1 phosphorylation, binds ceramide, and translocates to the plasma membrane where it regulates adherens and tight junction formation. PKC zeta contains an OPR domain (aa 15-98), a zinc finger region (aa 130-180), and a protein kinase domain (aa 252-518). PKC zeta potential variants of 409 or 539 aa have alternate start sites at aa 184 or aa 9 (also missing aa 186-233), respectively. Within the region used as an immunogen, human PKC zeta shares 98% aa identity with mouse and rat PKC zeta.
Long Name
Protein Kinase C zeta
Alternate Names
PKC2, PRKCZ
Gene Symbol
PRKCZ
UniProt
Additional PKC zeta Products
Product Documents for Human PKC zeta Antibody
Product Specific Notices for Human PKC zeta Antibody
For research use only
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