Human Plasminogen/Plasmin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2596
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 270412
Product Specifications
Immunogen
Human plasma-derived Plasminogen
Specificity
Detects human Plasminogen and Plasmin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human Plasminogen/Plasmin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Human Plasminogen (Catalog # 1939-SE), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Human Plasminogen (Catalog # 1939-SE) under non-reducing conditions only
Sample: Human Plasminogen (Catalog # 1939-SE) under non-reducing conditions only
Reviewed Applications
Read 1 review rated 4 using MAB2596 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plasminogen
Plasminogen (PLG) is the precursor of Plasmin, an active serine protease that dissolves the fibrin of blood clots and acts in many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion (1, 2). Synthesized in the kidney, Plasminogen is found in plasma and many extracellular fluids. Activated by u- or t-Plasminogen Activator, the single‑chain Plasminogen (aa 20-810) is converted to Plasmin, which consists of disulfide bond‑linked heavy chain A (aa 20-580) and light chain B (aa 581-810). Heavy chain A contains 5 kringle domains and light chain B corresponds to the serine protease domain. A fragment consisting of the first 4 kringle domains has been named Angiostatin, an angiogenesis inhibitor (3, 4).
References
- Petersen, T.E. et al. (1990) J. Biol. Chem. 265:6104.
- Forsgren, M. et al. (1987) FEBS Lett. 213:254.
- O’Reilly, M.S. et al. (1994) Cell 79:315.
- Sim, B.K. et al. (1997) Cancer Res. 57:1329.
Alternate Names
Plg
Gene Symbol
PLG
Additional Plasminogen Products
Product Documents for Human Plasminogen/Plasmin Antibody
Product Specific Notices for Human Plasminogen/Plasmin Antibody
For research use only
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