Human PLD2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10123
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 846224
Product Specifications
Immunogen
E. coli-derived recombinant human PLD2
Met1-His117
Accession # O14939
Met1-His117
Accession # O14939
Specificity
Detects huma PLD2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PLD2 Antibody
Detection of Human PLD2 by Western Blot.
Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PLD2 Monoclonal Antibody (Catalog # MAB10123) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PLD2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLD2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HCT‑116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line
Sample: HCT‑116 human colorectal carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, HUVEC human umbilical vein endothelial cells, U937 human histiocytic lymphoma cell line, and K562 human chronic myelogenous leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLD2
Phospholipase D2 (PLD2) is an enzyme encoded by the PLD2 gene. Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. The Phospholipase D (PLD) lipid-signaling enzyme superfamily has long been studied for its roles in cell communication and a wide range of cell biological processes. Potent PLD2 inhibitors are available, like VU 0364739 hydrochloride (Tocris Cat. 4171) and ML 298 hydrochloride (Tocris Cat. 4895). Three different PLD2 isoforms -PLD2A, PLD2B and PLD2C- have been described, ranging from 38 to 105 KDa.
Long Name
Phospholipase D2
Alternate Names
Choline Phosphatase 2, HPLD2, PLD1C
Gene Symbol
PLD2
UniProt
Additional PLD2 Products
Product Documents for Human PLD2 Antibody
Product Specific Notices for Human PLD2 Antibody
For research use only
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