Human Plexin B1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB37491A

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 559830

View all Plexin B1 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Plexin B1
Accession # O43157

Specificity

Detects human Plexin B1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Plexin B1 APC-conjugated Antibody

Detection of Plexin B1 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of Plexin B1 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Plexin B1 APC-conjugated Monoclonal Antibody (Catalog # FAB37491A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Plexin B1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Jurkat Human Acute T Cell Leukemia Cell Line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Plexin B1

Plexin B1 is a 300 kDa member of the B subfamily, Plexin family of Semaphorin receptors. Mature human Plexin B1 is a 2116 amino acid type I transmembrane (TM) glycoprotein that contains a 1471 aa extracellular domain (ECD) and a 612 aa cytoplasmic region. The ECD contains one Semaphorin domain, two PSI domains, and three IPT repeats. The ECD is cleaved into two subunits, a 200 kDa alpha-chain (aa 20-1305) and a 100 kDa TM beta-chain. The subunits are nondisulfide-linked and generate a high-affinity receptor. Plexin B1 is a receptor for Semaphorin 4D/CD100. It forms a receptor complex with neuropilins, MET, and EGF R2. Of three known splice variants, one shows a deletion of aa 689-871, a second shows a 53 aa substitution for aa 677‑2135, and a third shows a 53 aa substitution for the 53 aa between aa 677‑729. Over aa 35-150, human Plexin B1 shares 90% and 97% aa sequence identity with dog and mouse Plexin B1, respectively.

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Entrez Gene IDs

5364 (Human); 235611 (Mouse); 316009 (Rat)

Gene Symbol

PLXNB1

UniProt

O43157

Additional Plexin B1 Products

Product Documents for Human Plexin B1 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Plexin B1 APC-conjugated Antibody

For research use only

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