Human PLK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3804
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PLK1
Ala400-Ser603
Accession # P53350
Ala400-Ser603
Accession # P53350
Specificity
Detects human PLK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PLK1 Antibody
Detection of Human PLK1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human PLK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3804) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PLK1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line and Raji human Burkitt's lymphoma cell line
Reviewed Applications
Read 2 reviews rated 4.5 using AF3804 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLK1
Polo-like kinase-1 (PLK1) is a serine/threonine kinase that localizes to the mitotic spindle, and is involved in G2 and M phase cell cycle checkpoints. The expression of PLK1 is elevated in many different cancer types. Depletion of PLK1 in mammalian cells results in an inhibition of cell proliferation, decreased viability, and arrest in G2/M phase. PLK1 contains a polo-box domain that binds to phospho-serine and phospho-threonine residues.
Long Name
Polo-like Kinase 1
Alternate Names
STPK13
Gene Symbol
PLK1
UniProt
Additional PLK1 Products
Product Documents for Human PLK1 Antibody
Product Specific Notices for Human PLK1 Antibody
For research use only
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