Detects human PLZF in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) ZBTB38, rhZNF24, rhZNF143, rhZNF206, rhZNF281, or rhZNF423 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human PLZF APC-conjugated Antibody

Detection of PLZF in HL‑60 Human Cell Line by Flow Cytometry.

HL-60 human acute promyelocytic leukemia cell line was stained with Mouse Anti-Human PLZF APC-conjugated Monoclonal Antibody (Catalog # IC2944A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human PLZF APC-conjugated Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells
Sample: HL‑60 human acute promyelocytic leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: PLZF

Human PLZF is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc-finger domain. PLZF forms homodimers with RAR alpha and LAZ3 with its zinc-finger region. Alternate splice forms exist which are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. Human PLZF shares 96%, 97%, 96%, and 96% amino acid identity with rat, mouse, canine, and bovine PLZF, respectively.

Long Name

Promyelocytic Leukemia Zinc Finger

Alternate Names

ZBTB16, ZNF145

Entrez Gene IDs

7704 (Human); 235320 (Mouse)

Gene Symbol

ZBTB16

UniProt

Q05516

Additional PLZF Products

Product Documents for Human PLZF APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human PLZF APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human PLZF APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC2944A

Key Product Details

Species Reactivity

Validated:

Cited:

Applications