Human PMEL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11439
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1067144
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human PMEL
Lys25-Gln595
Accession # P40967
Lys25-Gln595
Accession # P40967
Specificity
Detects human PMEL in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PMEL Antibody
Detection of PMEL in Human Melanoma.
PMEL was detected in immersion fixed paraffin-embedded sections of Human Melanoma using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11439) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human PMEL Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Melanoma
Sample: Immersion fixed paraffin-embedded sections of Human Melanoma
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PMEL
References
- Zhang, S. Chen, K. Liu, H. Jing, C. Zhang, X. Qu, C. Yu, S. PMEL as a Prognostic Biomarker and Negatively
Associated With Immune Infiltration in Skin Cutaneous Melanoma (SKCM). J. Immunother. 2021 Jul-Aug 01;44(6):214-223.
doi: 10.1097/CJI.0000000000000374. PMID: 34028390; PMCID: PMC8225232. - Fan P.-D. Goff S.P. Mol. Cell. Biol. 20:7591-7601(2000). Chrystal, P.W. Footz, T. Hodges, E.D. Jensen, J.A. Walter, M.A. Allison, W.T. Functional Domains and Evolutionary
History of the PMEL and GPNMB Family Proteins. Molecules. 2021 Jun
9;26(12):3529.
doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697.
Long Name
Premelanosome Protein
Alternate Names
Gp100, ME20, ME20-M, ME20M, PMEL17, SILV
Gene Symbol
PMEL
UniProt
Additional PMEL Products
Product Documents for Human PMEL Antibody
Product Specific Notices for Human PMEL Antibody
For research use only
Loading...
Loading...
Loading...
Loading...