Human PMEL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11470
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 1067146
Product Specifications
Immunogen
Chinese Hamster Ovary cell line, CHO-derived human PMEL
Lys25-Gln595
Accession # P40967
Lys25-Gln595
Accession # P40967
Specificity
Detects human PMEL in direct ELISA.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PMEL Antibody
Detection of Human PMEL by Western Blot.
Western Blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11470) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for PMEL at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of PMEL in SK-MEL-28 cells (Positive) and K562 cells (Negative).
PMEL was detected in fixed SK-Mel-28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative) using Mouse Anti-Human PMEL Monoclonal Antibody (Catalog # MAB11470) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PMEL Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Fixed SK-Mel-28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative)
Sample: Fixed SK-Mel-28 human malignant melanoma cell line (Positive) and absent in K562 human chronic myelogenous leukemia cell line (Negative)
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line
Sample: A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PMEL
References
- Zhang, S. Chen, K. Liu, H. Jing, C. Zhang, X. Qu, C. Yu, S. PMEL as a Prognostic Biomarker and Negatively
Associated With Immune Infiltration in Skin Cutaneous Melanoma (SKCM). J. Immunother. 2021 Jul-Aug 01;44(6):214-223.
doi: 10.1097/CJI.0000000000000374. PMID: 34028390; PMCID: PMC8225232. - Fan P.-D. Goff S.P. Mol. Cell. Biol. 20:7591-7601(2000). Chrystal, P.W. Footz, T. Hodges, E.D. Jensen, J.A. Walter, M.A. Allison, W.T. Functional Domains and Evolutionary
History of the PMEL and GPNMB Family Proteins. Molecules. 2021 Jun
9;26(12):3529.
doi: 10.3390/molecules26123529. PMID: 34207849; PMCID: PMC8273697.
Long Name
Premelanosome Protein
Alternate Names
Gp100, ME20, ME20-M, ME20M, PMEL17, SILV
Gene Symbol
PMEL
UniProt
Additional PMEL Products
Product Documents for Human PMEL Antibody
Product Specific Notices for Human PMEL Antibody
For research use only
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