Human Podocalyxin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1658

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Virus

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot

Cited:

Cell Selection, ELISA Capture, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 222328

View all Podocalyxin Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Podocalyxin
Ser23-Arg427
Accession # AAB61574

Specificity

Detects human Podocalyxin in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Podocalyxin or recombinant human Endoglycan is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Podocalyxin Antibody

Detection of Podocalyxin antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of Podocalyxin in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells was stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.
Podocalyxin antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Podocalyxin in BG01V Human Stem Cells.

Podocalyxin was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Podocalyxin in Induced pluripotent stem cells (iPSCs) by Flow Cytometry

Induced pluripotent stem cells (iPSCs) were stained with Mouse Anti-Human Podocalyxin Monoclonal Antibody (Catalog # MAB1658, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Podocalyxin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: - BG01V human embryonic stem cells- Induced pluripotent stem cells (iPSCs)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Western Blot

1 µg/mL
Sample: Recombinant Human Podocalyxin
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.4 using MAB1658 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology (1). Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors (2). It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

References

  1. Li, J. et al. (2001) DNA Seq. 12:407.
  2. Hara, T. et al. (1999) Immunity 11:567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

AAB61574

Additional Podocalyxin Products

Product Documents for Human Podocalyxin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Podocalyxin Antibody

For research use only

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