MDA-MB-231 cells were stained with Mouse Anti-Human Porimin Monoclonal Antibody (Catalog # MAB3010, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Porimin Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Jurkat human acute T cell leukemia cell line, MDA‑MB‑231 human breast cancer cell line

Western Blot

1 µg/mL
Sample: Recombinant Human Porimin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Porimin

Porimin is a widely expressed mucin-related transmembrane glycoprotein. Cross-linking of Porimin leads to membrane disruption and cell death.

Long Name

Pro-Oncosis Receptor Inducing Membrane Injury

Alternate Names

KCT3, TMEM123

Entrez Gene IDs

114908 (Human)

Gene Symbol

TMEM123

UniProt

AAG21694

Additional Porimin Products

Product Documents for Human Porimin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Porimin Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Porimin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3010

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Porimin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Porimin Antibody

Detection of Porimin in MDA-MB-231 cells by Flow Cytometry.