Human POU3F2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7427
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 761340
Product Specifications
Immunogen
E. coli-derived recombinant human POU3F2
Asn7-His59
Accession # P20265
Asn7-His59
Accession # P20265
Specificity
Detects human POU3F2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity
with recombinant human (rh) POU5F1 or rhPOU4F3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human POU3F2 Antibody
Detection of Human POU3F2 by Western Blot.
Western blot shows lysates of A375 human melanoma cell line, Bowes human melanoma cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POU3F2 Monoclonal Antibody (Catalog # MAB7427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for POU3F2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human POU3F2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A549 human lung carcinoma cell line, Bowes human melanoma cell line, and SK‑Mel‑28 human malignant melanoma cell line
Sample: A549 human lung carcinoma cell line, Bowes human melanoma cell line, and SK‑Mel‑28 human malignant melanoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POU3F2
Long Name
POU Class 3 Homeobox 2
Alternate Names
Brain-2, BRN2, Oct7, OTF7
Entrez Gene IDs
5454 (Human)
Gene Symbol
POU3F2
UniProt
Additional POU3F2 Products
Product Documents for Human POU3F2 Antibody
Product Specific Notices for Human POU3F2 Antibody
For research use only
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