Human POU3F2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7427

R&D Systems, part of Bio-Techne
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MAB7427
MAB7427-SP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 761340

View all POU3F2 Antibodies »

Product Summary for Human POU3F2 Antibody

Immunogen

E. coli-derived recombinant human POU3F2
Asn7-His59
Accession # P20265

Specificity

Detects human POU3F2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) POU5F1 or rhPOU4F3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human POU3F2 Antibody

Detection of Human POU3F2 antibody by Western Blot.

Detection of Human POU3F2 by Western Blot.

Western blot shows lysates of A375 human melanoma cell line, Bowes human melanoma cell line, and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human POU3F2 Monoclonal Antibody (Catalog # MAB7427) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for POU3F2 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human POU3F2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line, Bowes human melanoma cell line, and SK‑Mel‑28 human malignant melanoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: POU3F2

POU3F2, also called Brn2, is a 47 kDa (predicted) member of the Octamer-binding transcription factor family. It is mainly expressed in the brain, especially in neuroectodermal cell lineages. With transcription factors ASCL1/Mash1 and Myt1L, POU3F2 has been used to induce neuronal differentiation of human stem cells and mouse fibroblasts. The full length transcript of POU3F2 produces the transcription factor N-Oct3, while alternate transcription initiation at amino acids 181 and 200 produce N-Oct5A and N-Oct5B isoforms, respectively. The sequence used as an immunogen is present only in N-Oct3 and is identical in human and mouse, while rat POU3F2 shows one amino acid difference.

Long Name

POU Class 3 Homeobox 2

Alternate Names

Brain-2, BRN2, Oct7, OTF7

Entrez Gene IDs

5454 (Human)

Gene Symbol

POU3F2

UniProt

P20265

Additional POU3F2 Products

Product Documents for Human POU3F2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human POU3F2 Antibody

For research use only

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