Human PP2C gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5595
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PP2C gamma
Met1-Asp546
Accession # O15355
Met1-Asp546
Accession # O15355
Specificity
Detects endogenous human PP2C gamma in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human PP2C gamma Antibody
Detection of Human PP2C gamma by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PP2C gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP2C gamma at approximately 72 to 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human PP2C gamma Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line
Sample: HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C gamma
PP2C gamma (Protein phosphatase 2C gamma; also PPM1G) is a nuclear member of the metal ion-dependent PP2C family of Ser/Thr protein phosphatases. Although its predicted MW is 59 kDa, it runs anomalously at 72‑75 kDa in SDS-PAGE. PP2C gamma is widely expressed and serves as a negative regulator of the cell stress response. In particular, it dephosphorylates histones H2A and H2B, allowing for their placement into chromatin. This is important when histones are reincorporated into DNA following DNA repair. Human PP2C gamma is 546 amino acids (aa) in length. It contains a catalytic domain (aa 27‑120), an Asp/Glu-rich region (aa 258‑326) and a poly-Lys tail (aa 539‑544). One potential splice form shows a Phe substitution for aa 20‑38.
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Gene Symbol
PPM1G
UniProt
Additional PP2C gamma Products
Product Documents for Human PP2C gamma Antibody
Product Specific Notices for Human PP2C gamma Antibody
For research use only
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