Human PPAR alpha/NR1C1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB24781
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2297B
Product Specifications
Immunogen
E. coli-derived recombinant human PPAR alpha/NR1C1
Met1-Asn99
Accession # Q07869
Met1-Asn99
Accession # Q07869
Specificity
Detects human PPAR alpha/NR1C1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human PPAR alpha/NR1C1 Antibody
PPAR alpha/NR1C1 in HT-29 Human Cell Line.
PPARa/NR1C1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rabbit Anti-Human PPARa/NR1C1 Monoclonal Antibody (Catalog # MAB24781) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PPAR alpha/NR1C1 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PPAR alpha/NR1C1
Peroxisome proliferator activated receptor alpha (PPARA) a member of the steroid hormone receptor superfamily, is a nuclear transcription factor which mediates the activity of peroxisome proliferators. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease.
Long Name
Peroxisome Proliferator-activated Receptor alpha
Alternate Names
NR1C1, PPARA
Entrez Gene IDs
5465 (Human)
Gene Symbol
PPARA
UniProt
Additional PPAR alpha/NR1C1 Products
Product Documents for Human PPAR alpha/NR1C1 Antibody
Product Specific Notices for Human PPAR alpha/NR1C1 Antibody
For research use only
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