Human Pro‑BNP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB36041
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 370124
Product Specifications
Immunogen
E. coli-derived recombinant human BNP
His27-His134
Accession # P16860
His27-His134
Accession # P16860
Specificity
Detects human Pro‑BNP in direct ELISAs. Does not detect BNP-32 (aa 103‑134).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Human Pro‑BNP Antibody
BNP in BG01V Human Cells.
BNP was detected in immersion fixed BG01V human embryonic stem cells using Rat Anti-Human Pro-BNP Monoclonal Antibody (Catalog # MAB36041) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Pro-BNP in Human Heart.
Pro-BNP was detected in immersion fixed paraffin-embedded sections of human heart using 25 µg/mL Rat Anti-Human Pro-BNP Monoclonal Antibody (Catalog # MAB36041) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to endothelial cells in the vasculature. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Pro‑BNP Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Western Blot
1 µg/mL
Sample: Human Pro-BNP
Sample: Human Pro-BNP
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BNP
Long Name
Brain Natriuretic Peptide
Alternate Names
BNF, NPPB
Gene Symbol
NPPB
UniProt
Additional BNP Products
Product Documents for Human Pro‑BNP Antibody
Product Specific Notices for Human Pro‑BNP Antibody
For research use only
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