Human Pro Coagulation Factor II/
Thrombin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1473
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 200710
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pro Coagulation Factor II/Thrombin
Ala44-Glu622
Accession # NP_000497
Ala44-Glu622
Accession # NP_000497
Specificity
Detects human Pro Coagulation Factor II/Thrombin in direct ELISAs and Western blots. It recognizes only Pro Coagulation Factor II/Thrombin, not active Thrombin. In Western blots, less than 5% cross-reactivity with recombinant human (rh) Factor X, rhKallikrein 1, 3, 5, and 11 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pro Coagulation Factor II/Thrombin Antibody
Detection of Human Pro Coagulation Factor II/Thrombin by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Pro Coagulation Factor II/Thrombin Monoclonal Antibody (Catalog # MAB1473) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Pro Coagulation Factor II/Thrombin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human Pro Coagulation Factor II/Thrombin Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Human Coagulation Factor II/Thrombin (Catalog # 2196-SE), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Human Coagulation Factor II/Thrombin (Catalog # 2196-SE), see our available Western blot detection antibodies
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Coagulation Factor II/Thrombin
Alternate Names
F2, Thrombin
Gene Symbol
F2
UniProt
Additional Coagulation Factor II/Thrombin Products
Product Documents for Human Pro Coagulation Factor II/Thrombin Antibody
Product Specific Notices for Human Pro Coagulation Factor II/Thrombin Antibody
For research use only
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