Human Pro Collagen I  alpha1 Antibody

Detection of Human Collagen I by Western Blot.

Western blot shows lysates of IMR-90 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human N-Pro Collagen I Monoclonal Antibody (Catalog # MAB6220) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Collagen I at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Pro-Collagen I alpha 1 in IMR‑90 Human Cell Line.

Pro-Collagen I alpha 1 was detected in immersion fixed IMR-90 human lung fibroblast cell line using Mouse Anti-Human Pro-Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB6220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Pro-Collagen I alpha 1 by Immunoprecipitation.

Human Pro-Collagen I alpha 1 was immunoprecipitated from 500 μg of IMR-90 human lung fibroblast cell line lysates with 5 μg of Mouse Anti-Human Pro-Collagen I alpha 1 Monoclonal Antibody (Catalog # MAB6220). The N-Pro Collagen I-antibody complexes were absorbed using Protein A or Protein G. Immunoprecipitated human Pro-Collagen I alpha 1 was detected by Western blot using 1 μg/mL of Sheep Anti-Human Pro-Collagen I alpha 1 Antigen Affinity-purified Polyclonal Antibody (AF6220) under reducing conditions and using Western Blot Buffer Group 1.