Human Pro- Collagen II Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB36151
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 819447
Product Summary for Human Pro- Collagen II Antibody
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Pro-Collagen II
Gln26-Arg266 and Gly957-Leu1487
Accession # P02458
Gln26-Arg266 and Gly957-Leu1487
Accession # P02458
Specificity
Detects human Pro-Collagen II in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Pro- Collagen II Antibody
Human Pro-Collagen II ELISA Standard Curve.
Recombinant Pro-Human Collagen II protein was serially diluted 2-fold and captured by Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB36151) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB3615) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Pro- Collagen II Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Pro-Collagen II Monoclonal Antibody (Catalog # MAB3615).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Pro-Collagen II DuoSet ELISA Kit (Catalog # DY7589-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collagen II
References
- Kuivaniemi H. et al. (1997) Hum. Mutat. 9:300.
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
UniProt
Additional Collagen II Products
Product Documents for Human Pro- Collagen II Antibody
Product Specific Notices for Human Pro- Collagen II Antibody
For research use only
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