Human Pro-Corticoliberin/Pro-CRH Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5805

R&D Systems, part of Bio-Techne
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AF5805
AF5805-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Pro-Corticoliberin/Pro-CRH Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pro‑Corticoliberin/Pro‑CRH
Leu25-Lys196
Accession # P06850

Specificity

Detects human Pro‑Corticoliberin/Pro‑CRH in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Pro-Corticoliberin/Pro-CRH Antibody

Detection of Human Pro-Corticoliberin/Pro-CRH antibody by Western Blot.

Detection of Human Pro-Corticoliberin/Pro-CRH by Western Blot.

Western blot shows lysates of human cerebellum and hypothalamus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Pro-Corticoliberin/Pro-CRH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5805) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-Corticoliberin/Pro-CRH at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Pro-Corticoliberin/Pro-CRH Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human cerebellum and hypothalamus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pro-Corticoliberin/Pro-CRH

Pro-Corticoliberin (also Pro-CRF and Pro-CRH) is a predicted 19 kDa member of the CRF/Urotensin I family of hormones. It is expressed by placental synctiotrophoblasts, zona fasciculata adrenal cells and paraventricular neurons, and is induced by glucocorticoids. Pro‑Corticoliberin is the precursor to a mature, 41 amino acid (aa) amidated Corticoliberin that induces pituitary ACTH and subsequent cortisol production by the adrenal. Human Pro‑Corticoliberin is 170 aa in length (aa 25‑194). If unprocessed, it undergoes N-terminal amidation to generate a polypeptide 168 aa in length (aa 27‑194). Secreted Pro‑Corticoliberin is both bioactive and binds to 37 kDa Corticoliberin-PB. In the placenta, Pro‑Corticoliberin is only occasionally cleaved to generate mature Corticoliberin (aa 152‑192); in the brain, it undergoes active internal proteolysis to generate multiple fragments, including the 5 kDa mature peptide. Over aa 25‑196, human Pro‑Corticoliberin shares 78% aa identity with mouse Pro‑Corticoliberin.

Long Name

Pro-Corticotropin-releasing Hormone

Alternate Names

CRF, CRH, ProCorticoliberin

Entrez Gene IDs

1392 (Human); 12918 (Mouse); 81648 (Rat)

Gene Symbol

CRH

UniProt

P06850

Additional Pro-Corticoliberin/Pro-CRH Products

Product Documents for Human Pro-Corticoliberin/Pro-CRH Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pro-Corticoliberin/Pro-CRH Antibody

For research use only

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