Human Procalcitonin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB83501
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 944002
Product Specifications
Immunogen
Human Procalcitonin synthetic peptide
Ala26-Asp38
Accession # P01258
Ala26-Asp38
Accession # P01258
Specificity
Detects human Procalcitonin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Procalcitonin Antibody
Detection of Human Procalcitonin by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Procalcitonin at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Procalcitonin in Human Thyroid.
Procalcitonin was detected in immersion fixed paraffin-embedded sections of human thyroid using Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Human Procalcitonin ELISA Standard Curve.
Recombinant Human Procalcitonin protein was serially diluted 2-fold and captured by Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83501) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Procalcitonin Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Procalcitonin Monoclonal Antibody (Catalog # MAB83502).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Procalcitonin DuoSet ELISA Kit (Catalog # DY8350-05) for convenient development of a sandwich ELISA.
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human thyroid
Sample: Immersion fixed paraffin-embedded sections of human thyroid
Western Blot
1 µg/mL
Sample: TT human medullary thyroid cancer cell line
Sample: TT human medullary thyroid cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Procalcitonin
Human Procalcitonin (CALCA and Calcitonin) is 141 amino acids (aa) in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-38, human Procalcitonin shares 64% aa identity with mouse Procalcitonin.
Alternate Names
CALC1, CALCA, Calcitonin 1, calcitonin gene-related peptide 1, CGRP, Katacalcin
Gene Symbol
CALCA
UniProt
Additional Procalcitonin Products
Product Documents for Human Procalcitonin Antibody
Product Specific Notices for Human Procalcitonin Antibody
For research use only
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