Human Prolyl Oligopeptidase/PREP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4308
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PREP
Leu2-Pro710
Accession # P48147
Leu2-Pro710
Accession # P48147
Specificity
Detects human PREP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PREP is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Human Prolyl Oligopeptidase/PREP Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Prolyl Oligopeptidase/PREP (Catalog # 4308‑SE), see our available Western blot detection antibodies
Sample: Cell lysates spiked with Recombinant Human Prolyl Oligopeptidase/PREP (Catalog # 4308‑SE), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Prolyl Oligopeptidase/PREP (Catalog # 4308-SE)
Sample: Recombinant Human Prolyl Oligopeptidase/PREP (Catalog # 4308-SE)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Prolyl Oligopeptidase/PREP
Prolyl Oligopeptidase is a serine peptidase displaying specificity for the cleavage of Pro-Xaa bonds of oligopeptide substrates (1, 2). The peptidase is known to hydrolyze a variety of biologically active peptides such as bradykinin, substance P, neurotensin, and vasopressin (3). Because of its action on neuropeptides, Prolyl Oligopeptidase is considered to be involved in processes such as learning, memory, and depression (4).
References
- Tarrago, T. et al. (2005) J. Pept. Sci. 11:283.
- Yoshimoto, T. et al. (1977) Biochemistry. 16:2942.
- Wilk, S. (1983) Life Sci. 33:2149.
- Maes, M. et al. (1994) Biol. Psychiatry. 35:545.
Alternate Names
PE, PREP, rPop
Gene Symbol
PREP
UniProt
Additional Prolyl Oligopeptidase/PREP Products
Product Documents for Human Prolyl Oligopeptidase/PREP Antibody
Product Specific Notices for Human Prolyl Oligopeptidase/PREP Antibody
For research use only
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