Prominin 2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Prominin 2 Monoclonal Antibody (Catalog # MAB2024) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Prominin 2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: LNCaP human prostate cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC-3 human prostate cancer cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prominin 2

Prominin 2 is a pentaspan membrane glycoprotein predominantly expressed in neuroepithelial cells, hematopoietic stem cells and epithelial cells of the adult kidney and digestive tract (1, 2). Prominin 2 is a 112-kDa glycoprotein structurally related to prominin 1 (CD133). The amino acid identity between prominin 1 and prominin 2 is low (<30%), but they exhibit some redundant functions.

References

Fargeas, C.A. et al. (2003) J. Biol. Chem. 278(10):8586.
Fargeas, C.A. et al. (2003) Stem Cells 21(4):506.

Alternate Names

PROM2, PROML2

Entrez Gene IDs

150696 (Human); 192212 (Mouse); 192211 (Rat)

Gene Symbol

PROM2

UniProt

AAM10541

Additional Prominin 2 Products

Product Documents for Human Prominin 2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Prominin 2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Prominin 2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2024

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Prominin 2 Antibody

Prominin 2 in HepG2 Human Cell Line.