Human Proprotein Convertase 9/PCSK9 (Research Grade Alirocumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10347
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu135
Product Specifications
Immunogen
Human Proprotein Convertase 9/PCSK9
Specificity
Detccts Human Proprotein Convertase 9/PCSK9 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Alirocumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images
Detection of Proprotein Convertase 9/PCSK9 in HeLa human carcinoma cell line by Flow Cytometry.
HeLa human cervical carcinoma cell line was stained with Human Anti-Human Proprotein Convertase 9/PCSK9 (Research Grade Alirocumab Biosimilar) Monoclonal Antibody (Catalog # MAB10347, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.Applications
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Sample: HeLa human carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Sample: HeLa human carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Proprotein Convertase 9/PCSK9
Alternate Names
FH3, FHCL3, HCHOLA3, LDLCQ1, NARC-1, NARC1, PC9, PCSK9
Gene Symbol
PCSK9
Additional Proprotein Convertase 9/PCSK9 Products
- All Products for Proprotein Convertase 9/PCSK9
- Proprotein Convertase 9/PCSK9 cDNA Clones
- Proprotein Convertase 9/PCSK9 ELISA Kits
- Proprotein Convertase 9/PCSK9 Lysates
- Proprotein Convertase 9/PCSK9 Primary Antibodies
- Proprotein Convertase 9/PCSK9 Proteins and Enzymes
- Proprotein Convertase 9/PCSK9 Simple Plex
Product Specific Notices
For research use only
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