Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB64371
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 737512
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protein O‑Glucosyltransferase 1/POGLUT
Arg24-Leu388
Accession # Q8NBL1
Arg24-Leu388
Accession # Q8NBL1
Specificity
Detects human Protein O‑Glucosyltransferase 1/POGLUT in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody
Protein O‑Glucosyltransferase 1/POGLUT in Human Liver.
Protein O-Glucosyltransferase 1/POGLUT was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Protein O-Glucosyltransferase 1/POGLUT Monoclonal Antibody (Catalog # MAB64371) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver
Sample: Immersion fixed paraffin-embedded sections of human liver
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protein O-Glucosyltransferase 1/POGLUT1
Protein O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). POGLUT1 has hydrolase activity on UDP-Glc in a phosphatase-coupled glycosyltransferase assay (4).
References
- Acar, M. et al. (2008) Cell 132:247.
- Teng, Y. et al. (2006) Gene 371:7
- Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
- Wu, Z.L. et al. (2011) Glycobiology 21:727.
Alternate Names
CLP46, KDELCL1, KTELC1, MDS010, MDSRP, POGLUT1, Rumi
Entrez Gene IDs
56983 (Human)
Gene Symbol
POGLUT1
UniProt
Additional Protein O-Glucosyltransferase 1/POGLUT1 Products
Product Documents for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody
Product Specific Notices for Human Protein O-Glucosyltransferase 1/POGLUT1 Antibody
For research use only
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