Human Proteinase 3/Myeloblastin/PRTN3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6134

R&D Systems, part of Bio-Techne
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AF6134
AF6134-SP
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Key Product Details

Species Reactivity

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Proteinase 3/Myeloblastin/PRTN3 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Proteinase 3/Myeloblastin/PRTN3
Ala26-Arg249
Accession # P24158

Specificity

Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Detection of Human Proteinase 3/Myeloblastin/ PRTN3 antibody by Western Blot.

Detection of Human Proteinase 3/Myeloblastin/ PRTN3 by Western Blot.

Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes (PBL), and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Proteinase 3/Myeloblastin/ PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Human Proteinase 3/Myeloblastin/PRTN3 antibody by Simple WesternTM.

Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Simple WesternTM.

Simple Western lane view shows lysates of human peripheral blood mononuclear cell tissue, loaded at 0.2 mg/mL. A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human peripheral blood mononuclear cell tissue

Western Blot

1 µg/mL
Sample: HL‑60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes, and human peripheral blood mononuclear cells

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proteinase 3/Myeloblastin/PRTN3

Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

Alternate Names

ACPA, AGP7, C-ANCA, MBN, MBT, Myeloblastin, NP4, PRTN3

Entrez Gene IDs

5657 (Human); 19152 (Mouse); 314615 (Rat)

Gene Symbol

PRTN3

UniProt

P24158 (Human)

Additional Proteinase 3/Myeloblastin/PRTN3 Products

Product Documents for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

For research use only

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