Human Protogenin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6795
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 814403
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Protogenin
Phe36-Ala952
Accession # Q2VWP7
Phe36-Ala952
Accession # Q2VWP7
Specificity
Detects human Protogenin in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human Protogenin Antibody
Adhesion Induced by Protogenin and Neutralization by Human Protogenin Antibody.
Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND50 is typically 0.4-2.4 µg/mL.Applications for Human Protogenin Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize Protogenin-induced adhesion in the P19 mouse embryonal carcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2.4 µg/mL in the presence of 10 µg/mL Recombinant Human Protogenin.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protogenin
(aa 36-952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36-411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG.
Alternate Names
IGDCC5, PRTG, RPTG, Shen-Dan
Gene Symbol
PRTG
UniProt
Additional Protogenin Products
Product Documents for Human Protogenin Antibody
Product Specific Notices for Human Protogenin Antibody
For research use only
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