Human PTGER1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10008
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 998412
Product Specifications
Immunogen
Synthetic peptide containing human PTGER1
Accession # P34995
Accession # P34995
Specificity
Detects human PTGER1 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PTGER1 Antibody
PTGER1 in Human Breast Cancer Tissue.
PTGER1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human PTGER1 Monoclonal Antibody (Catalog # MAB10008) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human PTGER1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Reviewed Applications
Read 1 review rated 5 using MAB10008 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTGER1
Prostaglandin E2 Receptor 1/ PTGER1/EP1, a member of the G protein-coupled receptor family, is one of four receptors for Prostaglandin E2 (PGE2). Expression is highest in kidney and spleen, with very low expression in most other tissues. Stimulation of angiogenesis in primate ovulatory follicles by luteinizing hormone has been shown to be mediated by PGE2 through PTGER1 and PTGER2 receptors. In epidermis, E2 plays a role in keratinocyte differentiaton. Expression and function of EP1 in carcinomas has been studied. For example, cytoplasmic and nuclear localization of EP1 in breast cancer has been reported in association with prognostic markers. And activation of EP1 mediated by COX2 causes VEGFC upregulation in lung adenocarcinomas.
Long Name
Prostaglandin E2 Receptor 1, Subtype EP1
Alternate Names
EP1
Gene Symbol
PTGER1
UniProt
Additional PTGER1 Products
Product Documents for Human PTGER1 Antibody
Product Specific Notices for Human PTGER1 Antibody
For research use only
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