Human PTGER3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB102431
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 1026603
Product Specifications
Immunogen
Human PTGER3 synthetic peptide
Accession # P43115
Accession # P43115
Specificity
Detects human PTGER3 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PTGER3 Antibody
Detection of Human PTGER3 by Western Blot.
Western blot shows lysates of HDLM-2 human Hodgkin’s lymphoma cell line and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB102431) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for PTGER3 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
PTGER3 in Human Kidney Tissue.
PTGER3 was detected in immersion fixed paraffin-embedded sections of human kidney tissue using Mouse Anti-Human PTGER3 Monoclonal Antibody (Catalog # MAB102431) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human PTGER3 Antibody
Application
Recommended Usage
Immunohistochemistry
0.1-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney tissue
Sample: Immersion fixed paraffin-embedded sections of human kidney tissue
Western Blot
2 µg/mL
Sample: HDLM‑2 human Hodgkin's lymphoma cell line and human kidney tissue
Sample: HDLM‑2 human Hodgkin's lymphoma cell line and human kidney tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTGER3
Human Prostaglandin EP3 receptor, also known as EP3 or PTGER3, is a 53kDa prostaglandin receptor for prostaglandin E2 (PGE2) encoded by the PTGER3 gene. PTGER3 is a G protein-coupled receptor (GPCR) of the rhodopsin-like receptor family. Along with several other receptor subtypes (EP1, EP2, and EP4), it mediates the diverse biological effects of Prostaglandin E2 (PGE2). PGE2 has been implicated in numerous processes, including immune response modulation, male erectile function, induction of labor, cervical cancer, control of blood pressure, and asthma. Various isoforms are produced by alternative splicing. Prostaglandin E2 Receptor EP3 expression has been documented throughout the periphery, especially kidney. ESTs have been isolated primarily from kidney libraries.
Long Name
Prostaglandin E2 Receptor 3, Subtype EP3
Alternate Names
EP3
Gene Symbol
PTGER3
UniProt
Additional PTGER3 Products
Product Documents for Human PTGER3 Antibody
Product Specific Notices for Human PTGER3 Antibody
For research use only
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